Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Protective role of allicin (diallyl thiosulfinate) on cell surface glycoconjugate moieties in 7,12-dimethylbenz(a) anthracene-induced oral carcinogenesis

Sasikumar Dhanarasu

Department of Biochemistry, College of Medicine, University of Hail, PO Box 2440, Hail, Kingdom of Saudi Arabia;

For correspondence:- Email: drdskumar31@yahoo.com Tel:+966535461930

Received: 5 July 2015 Accepted: 20 April 2017 Published: 31 August 2017

Citation: Dhanarasu S. Protective role of allicin (diallyl thiosulfinate) on cell surface glycoconjugate moieties in 7,12-dimethylbenz(a) anthracene-induced oral carcinogenesis. Trop J Pharm Res 2017; 16(8):1797-1805 doi: 10.4314/tjpr.v16i8.7

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the protective and restoring effects of allicin in 7,12-dimethylbenz(a)anthracene (DMBA)-induced buccal pouch carcinogenesis.

Methods: 1 week after receiving allicin (20 mg/kg body weight) orally, the buccal pouches of hamsters were painted daily with 0.5% 7,12-dimethylbenz(a)anthracene (DMBA) in liquid paraffin for 14 weeks and then every other day for another 2 weeks, after receiving allicin orally and thereafter for 14 weeks. The protective effects of allicin was evaluated by measuring the tumour incidence, tumour volume and tumour burdens as well as the levels of glycoconjugates were analyzed by using specific colorimetric methods. Animals not exposed to allicin and/or DMBA, those exposed to DMBA alone and others exposed to allicin alone served as controls.

Results: Allicin significantly reduced the tumour incidence, tumour volume and tumour burden. DMBA-altered glycoconjugates in plasma, buccal mucosa tumour tissues and erythrocyte membrane of tumour bearing hamsters were normalized after treated with allicin.

Conclusion: The results suggest that allicin has considerable potential to protect and restore the cell surface glycoconjugates moieties in the presence of allicin or possibly other oral carcinogenic agents.

Keywords: Oral cancer, 7,12-dimethylbenz(a)anthracene, cell surface glycoconjugates, allicin, diallyl thiosulfinate